| Seed Production and
Quality - 87 |
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Project Leader and Principal UC Investigators Kent J. Bradford |
A seed bank has been established to provide seed lots of varying vigor that
can be used to 1) identify laboratory tests which can separate seed lots
according to some criterion, and 2) determine whether the results of the
laboratory tests predict performance in the field. A number of tests, including germination, rate of germination, seedling length, and accelerated aging are being evaluated. A number of variations of an accelerated aging test were screened. The test is based on the principle that as seeds deteriorate, vigor declines first, followed by loss of viability. By placing seeds in extremely adverse conditions of high temperature and high moisture content, aging equivalent to several years of normal storage can be induced within a few days or even hours. The usefulness of the accelerated aging test is that it can bring out differences among lots which differ little on the standard germination test. Germination after aging should give a better ranking of seed lots for vigor than does germination before aging. The aging tests generally identified the lowest quality seed lots, but germination after aging was not always correlated with the initial germination percentage. Whether accelerated aging tests can predict field performance still needs to be determined. Correlation studies were done among various quality characteristics for M-201. All correlations with seedling length were negative, indicating an inverse correlation of seedling length with the other quality characteristics. Testing procedures may have caused this unexpected relationship. Duration of future tests will be shortened. Field emergence trials need to be evaluated before a firm decision can be made regarding which test, or tests, will give the best indication of potential field performance. Moisture Effects on Seed Quality ExploredThe relationships between seed maturity, seed moisture content at harvest and seed quality are being studied for M-201 and L-202. Seed lots have been harvested at a range of moisture contents and are currently being evaluated to determine 1) whether seed vigor and quality continue to increase as moisture content declines to very low levels, and 2) whether seed maturity or moisture content is the more critical factor for seed quality. Seed Priming Has Potential for RiceThe idea behind seed priming is to osmotically restrict germination to allow all seeds to reach the same stage, then release the inhibition, giving rapid and uniform germination. Studies have established the ranges of osmotic potentials needed to manipulate rice seed germination. Increasing the osmotic concentration of mannitol in the germination medium successively delayed and prevented germination. Much less osmoticum is needed to delay germination than is needed to completely stop it. Since rice seed is soaked before planting, these procedures could be incorporated into the soaking period. Another potential application would be to block germination in soaked seeds if bad weather prevents planting on schedule.
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